Anti-inflammatory and antioxidant activity of Joshanda partially mediated through inhibition of lipoxygenase

Joshanda, a polyherbal product is commonly used to treat inflammation of the mucous membranes of nose and air passages. The current study was planned to evaluate the antiinflammatory and antioxidant activity of Decoction of Joshanda (DJ). DJ was challenged in carrageenan induced paw edema and cotton pellets induced granuloma at 100, 200 and 300 mg/kg i.p. for its antiinflammtory profile. However, for its antioxidant activity, DDPH free radical scavenging test was used. DJ manifested marked attenuation of edema (56%) induced by carrageenan, at 300 mg/kg after 4 hour of administration. In cotton pellet induced granuloma, DJ exhibited profound reduction in granuloma formation which was 52.74% at 300 mg/kg i.p. respectively. When tested against lipoxygenase, DJ illustrated profound inhibition (55% at 100 μg/ml) in a concentration dependent manner. Against DDPH free radical, DJ elicited prominent scavenging activity (58%) and thus strongly harmonized the antiinflammatory activity. Our findings suggest that DJ strongly ameliorated the induced inflammation at least partially mediated through lipoxygenase inhibition and thus the results were consistent with the traditional uses of Joshanda as an antiinflammatory agent.